HPLC Method Validation for Simultaneous Determination of Three Mycotoxins from Corn Seeds

摘要

A new HPLC optimized method for simultaneous determination of some mycotoxins by separationand using a Dionex UltiMate3000 modular system, with multichannel UV detector was developedand validated. The method optimization was performed to determine simultaneously, several relevant mycotoxinsfrom corn seeds that were stored over 8 months. The mycotoxins selected for quantificationwere: aflatoxin B1, ochratoxin A and zearalenone. They were selected due to their high concentration inthe fodder. For the development stages of the HPLC method, it was introduced an internal standard tohave accurate results. Taking into account that the analytes should be extracted from the complex matrixthey reside, an extraction procedure was performed, using organic solvents, and the selection afterrepeated tests demonstrated the best capacity for ethyl acetate. The recovery is about 60–70 % after theextraction process also there is a good preconcentration (×2.5) of the analytes and the internal standard fortheir quantification.The limit of quantification (LOQ) obtained by chromatographic parameters optimization, for aflatoxinB1 and ochratoxin A were about 3–5 μg kg–1 and 14.4 μg kg–1 for zearalenone of raw biologicalmaterial, making these values lower than those accepted by the actual normatives and regulations.(doi: 10.5562/cca1788)